Domain architecture of p53 and nomenclature of clones generated via CRISPR-Cas9. (A) Highlights the domain architecture of p53 with emphasis on the sgRNA designed to target the oligomerization (OD) and C-terminal domain (CTD) of mtp53. (B) CRISPR-Cas9 was used to generate clones with either OD or CTD mutations. Clones were selected with FACS sorting of eGFP positive cells. Selected clones were named based on the region and type of mutation that resulted.
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